Use of an arbitrarily primed PCR product in the development of a Campylobacter jejuni-specific PCR
نویسندگان
چکیده
منابع مشابه
Cloning and expression of the Campylobacter jejuni lon gene detected by RNA arbitrarily primed PCR.
Fingerprinting of RNA by arbitrarily primed PCR was used to identify a heat-inducible gene in Campylobacter jejuni. Comparing RNA fingerprints from C. jejuni cells before and after 20 min of heat shock at 48 degrees C, a differentially amplified PCR product was identified which displayed a high degree of homology to bacterial lon genes. By screening C. jejuni genomic libraries, the entire lon g...
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The polysaccharide capsule (CPS) of Campylobacter jejuni is a virulence factor linked to cell surface carbohydrate diversity which mainly determines the serotypes. Thirty-four CPS gene cluster structures have been published and some of them can be distinguished by multiple-PCR. Penner serotypes HS1/44c, HS2, HS4c, HS19, HS23/36c and HS41 are markers for Guillain-Barré syndrome (GBS). The capsul...
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for functional analysis. Mutagenesis to the small promoter-only containing plasmid is preferred to that of the reporter gene construct, because amplification of a smaller plasmid is faster and more efficient and removes the slight possibility of introducing random mutations to the reporter gene. The transferred mutant region can be sequenced to confirm that no random mutations were introduced d...
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Clostridium difficile is now recognized as the major agent responsible for nosocomial diarrhea in adults. Among the genotyping methods available, arbitrarily primed PCR (AP-PCR), PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) have been widely used for investigating outbreaks of C. difficile infections. However, the comparative typing ability, reproducibility, discriminatory power, ...
متن کاملDNA fingerprinting of mammalian cell lines using nonradioactive arbitrarily primed PCR (AP-PCR).
probe. Retention of the probe on the dialysis bag using the electroelution protocol and inefficient binding of the probe to glass beads (as seen with ds DNA below 0.5 kb in size) contribute to probe losses. Since the ss probe does not need to be purified from the LMP agarose gel, loss of the probe associated with the two established protocols described here (particularly with the glass bead pro...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1997
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.63.3.1019-1023.1997